Journal: Cell

Article Title: Neuromodulator Signaling Bidirectionally Controls Vesicle Numbers in Human Synapses

doi: 10.1016/j.cell.2019.09.011

Figure Lengend Snippet: Highlights:

Article Snippet: FUW-TetO-EGFP (lentiviral vector) , Vierbuchen et 2010 , 30130 Addgene.

Techniques: Transduction, Recombinant, Plasmid Preparation, Expressing, Software

Journal: Biology

Article Title: A Novel Vitronectin Peptide Facilitates Differentiation of Oligodendrocytes from Human Pluripotent Stem Cells (Synthetic ECM for Oligodendrocyte Differentiation)

doi: 10.3390/biology10121254

Figure Lengend Snippet: Induction and expansion of hPSC-derived OPCs. ( A ) A bright-field image of OPCs at day 12 of differentiation. Scale bar: 20 µm. (Inset) A representative image of NESTIN-positive cells in the culture on day 12. ( B , C ) Immunofluorescent images of OLIG2, A2B5 and NG2. ( D ) Quantification of cells positive for OLIG2, A2B5, and NG2. ( E ) Quantitative gene expression of OPC markers ( OLIG2 and PDGFRα ) and a pluripotency marker ( NANOG ). ( F , G ) Representative images of OLIG2, A2B5- and NG2-positive cells after multiple passages (at passage 11) accompanied with a freeze-thawing cycle. Scale bar: 20 µm. ( H ) Quantification of marker-positive cells at passage 11. ( I ) Quantitative gene expression of OPC markers ( PDGFRα and SOX10 ). Scale bars: 10 µm. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, Student t -test.

Article Snippet: One day later, the cells were infected with a lentivirus encoding SOX10 (Addgene, clone ID number 45843, Watertown, MA, USA) and a lentivirus of reverse tetracycline-controlled transactivator ( rtTA ) (Addgene, clone ID number 20342) for doxycycline (DOX)-induced SOX10 expression and incubated for 14–16 h. Viral vectors and packaging were constructed as described elsewhere [ ].

Techniques: Derivative Assay, Gene Expression, Marker

Journal: Biology

Article Title: A Novel Vitronectin Peptide Facilitates Differentiation of Oligodendrocytes from Human Pluripotent Stem Cells (Synthetic ECM for Oligodendrocyte Differentiation)

doi: 10.3390/biology10121254

Figure Lengend Snippet: Differentiation of oligodendrocytes from hPSC-derived OPCs via SOX10 overexpression. ( A ) A schematic diagram for oligodendrocyte differentiation from hPSC-derived OPCs. ( B – D ) Time-course expression of OPC markers during differentiation. As differentiation proceeded, the transcript levels of OLIG2 and PDGFR-α gradually decreased ( C , D ) whereas that of SOX10 was maintained due to continued exogenous expression ( B ). ( E – G ) Time-course expression of OD markers during differentiation. Expression levels of all marker genes tested were upregulated by SOX10-induced differentiation and plateaued after day 8. ( H , I ) Representative image of O4- and MBP-positive cells induced by the overexpression of SOX10. Scale bars: 12.5 µm. ** p < 0.01, **** p < 0.0001, one-way ANOVA with Dunnett’s post hoc test.

Article Snippet: One day later, the cells were infected with a lentivirus encoding SOX10 (Addgene, clone ID number 45843, Watertown, MA, USA) and a lentivirus of reverse tetracycline-controlled transactivator ( rtTA ) (Addgene, clone ID number 20342) for doxycycline (DOX)-induced SOX10 expression and incubated for 14–16 h. Viral vectors and packaging were constructed as described elsewhere [ ].

Techniques: Derivative Assay, Over Expression, Expressing, Marker

Journal: Biology

Article Title: A Novel Vitronectin Peptide Facilitates Differentiation of Oligodendrocytes from Human Pluripotent Stem Cells (Synthetic ECM for Oligodendrocyte Differentiation)

doi: 10.3390/biology10121254

Figure Lengend Snippet: Screening for ECM materials facilitating oligodendrocyte differentiation induced by SOX10. ( A – F ) Representative images of O4- and MBP-positive cells generated on the indicated matrix. White arrows are pointing to double-positive cells for O4 and MBP. ( G , H ) Quantification of the number of O4- or MBP-positive cells on day 12 after initiation of oligodendrocyte maturation on the indicated substrates. * p < 0.05, ** p < 0.01, one-way ANOVA with Tukey’s post-hoc test.

Article Snippet: One day later, the cells were infected with a lentivirus encoding SOX10 (Addgene, clone ID number 45843, Watertown, MA, USA) and a lentivirus of reverse tetracycline-controlled transactivator ( rtTA ) (Addgene, clone ID number 20342) for doxycycline (DOX)-induced SOX10 expression and incubated for 14–16 h. Viral vectors and packaging were constructed as described elsewhere [ ].

Techniques: Generated

Journal: Cell reports

Article Title: KLF4, A Gene Regulating Prostate Stem Cell Homeostasis, Is a Barrier to Malignant Progression and Predictor of Good Prognosis in Prostate Cancer

doi: 10.1016/j.celrep.2018.11.065

Figure Lengend Snippet: KEY RESOURCE TABLE

Article Snippet: Lentiviral Dox inducible BFP-tagged human KLF4 expressing vector was constructed by modifying the lentiviral vector FUW-tetO-hKLF4 generated by Rudolf Jaenisch ( www.addgene.org ).

Techniques: Plasmid Preparation, Recombinant, SYBR Green Assay, Transformation Assay, shRNA, Software